Taq PCR Master Mix can be stored at 2–8☌ for up to 2 months , allowing even faster PCR setup by eliminating thawing time. Due to the convenient master mix format, pipetting errors are minimized, ensuring highly reproducible PCR results (see figure " Reproducible PCR"). Only primers and template DNA need to be added to set up PCR.
This ready-to-use solution also includes the QIAGEN PCR Buffer, MgCl 2, and ultrapure dNTPs at optimized concentrations. The Taq PCR Master Mix Kit includes QIAGEN's Taq DNA Polymerase in a premixed format. Substrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent-dNTP/ddNTP Due to the unique PCR buffer included in the master mix, optimization of PCR by varying the annealing temperature or the Mg 2+ concentration is dramatically reduced and often not required (see figures " Wide annealing temperature window" and " Tolerance to variable magnesium concentration").
Taq DNA Polymerase ensures highly specific amplification with different primer–template systems (see figures " Tolerance of different primer T m values" and " Specific amplification of long PCR products"). Every lot of QIAGEN's Taq DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified from human genomic DNA (see figure " Lot-to-lot reproducibility"). The Taq PCR Master Mix Kit outperformed kits tested from other suppliers and ensures reliable PCR performance in a wide range of PCR applications - without the need for time-consuming optimization (see figure " Reproducible PCR").
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